سلولی و مولکولی

در کنار خدمات تخصصی کلان، شرکت لیوژن فارمد قادر است تا کلیه خدمات در حوزه­ های مختلف ژن،سلول،پروتئین،ویروس،میکروب و … را به هر صورتی که مورد نیاز مشتری باشد، ارائه دهد. همچنین با توجه به اهمیت پروتئین­­ ها در صنایع گوناگون مانند صنایع دارویی، صنایع غذایی، شوینده و… نیاز به مطالعات تخصصی بر روی پروتئین­های گوناگون از اهمیت بالایی برخوردار است. آزمون­ هایی جهت شناسایی، تعیین غلظت، خالص­ سازی، مطالعات فیزیکی و شیمیایی و… از جمله این مطالعات می باشند. شرکت لیوژن فارمد با در اختیار داشتن تجهیزات مناسب، توانایی انجام تکنیک های متنوع در مطالعات پروتئین ها و متابولیت­ های متنوع را دارا می­باشد.

Molecular Services

Reverse Transcriptase PCR

All types of PCR

Gel Documentation

Real Time PCR

Determination of Concentration and Purity of Nucleic Acids with Nanodrop reader

Horizontal / Vertical electrophoresis

cDNA Synthesis

Spectrophotometer (200-800nm)

Plasmid, DNA and RNA Extraction

Gene Cloning

Molecular Phylogeny Study via DNA Barcoding

Genome Methylation Analysis

Sequencing Results Analysis

DNA, RNA Sequencing

High Resolution Melting (HRM)

Gene Expression Analysis by Real-Time PCR (Relative & Absolute)

 

Cellular Services

­­­­­ Scratch Assay

Fluorescent Microscope

Genotoxicity Analysis Micronucleus Assay

Evaluation of Substance Toxicity on Cell Lines By MTT, XTT Test

Cell Transfection With RNA, DNA

Virus Packaging And Titration

Cell-Based Immunogenicity

Transduction

Flow Cytometry

Migration Assay

Cell Counting

Cell Culture, Cell Expansion, And Cell Banking

Primary Stem Cell Culture And Their Differentiation

Cell Viability

Ipsc Production

Cells Immortalization

Cell-Based Binding Assay

Evaluation of The Cell Growth Profile

Mab Cell-Based Potency And Bioassay

ELISA-Based Binding Assay

Detection Of Mycoplasma Cell Culture Samples By DNA Staining

Potency of Biotech Drugs Products

Mycoplasma Detection By qPCR

Cell Treatment With Herbal Extracts

Detection Of Bacterial / Fungal Contamination By Culture Method

Detection of Mycoplasma Contamination By Specific Culture And Indicator Cell

Antigen, Receptor or Ligand Density (Such As Binding Assays And Competitive Binding Assays)

Qualitative Detection of Viral/ Bacteria Contamination By Real-Time PCR

Apoptosis / Survival

Intracellular Expression of Proteins

Cell Cycle Analysis

Enzymatic Activity

Changes In pH, Intracellular Calcium And Glutathione

Target Specific Reporter Bioassays

Karyotyping

Cell And Tissue Staining

 

Viral Services

Virus Titration by TCID50, HA and qPCR

Bovine, Porcine and Human virus Detection qPCR

HA and HAD

TEM analysis

Evaluation of Antiviral Properties of Detergents and Solutions

Microbial Services

CFU/Gram Staining

Mycoplasma Detection

Microbial and Fungal Detection

Bacteriophage Detection

MIC/MBC/Biofilm Assay

TSA/SDA/5 Days monitoring

Microbial Isolation Identification in Water and Food

Microbial Culture and Differentiation Detection test (urease-TSI-SIM-Peroxidase-Catalase -Oxidase- Coagulase)

Protein services

Two-Dimensional Electrophoresis

Amino Acid Analysis(AAA)

High Performance Liquid Chromatography (HPLC)

Isoelectric Focusing Electrophoresis

Gas Chromatography

Protein Liquid Chromatography (FPLC)

Ultrasonic

Western Blotting

SDS-PAGE/PAGE

UV–visible Spectrophotometry

Hygrometry by Humidity meter

ELISA

Enzymatic studies

Turbidity Measurement

Protein Extraction and Purification

Cell-based Biological Assays

Heavy Metal Measurement

Protein Concentration (by Bradford, lowry and etc.)

Chemical Analysis

Acid-Base Titration

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