{"id":823,"date":"2019-12-03T08:14:59","date_gmt":"2019-12-03T09:14:59","guid":{"rendered":"http:\/\/livogen.co\/?p=823"},"modified":"2019-12-07T05:58:14","modified_gmt":"2019-12-07T06:58:14","slug":"hcd-extraction-kit","status":"publish","type":"post","link":"https:\/\/livogen.co\/fa\/2019\/12\/03\/hcd-extraction-kit\/","title":{"rendered":"HCD Extraction Kit"},"content":{"rendered":"\n
\n
\n

Quantity<\/strong>: 50 reactions Buffer
Storage <\/strong>: 2-8 \u00b0C
Shipment<\/strong>: Ice gel
Lysis Mix Storage<\/strong>: -20 \u00b0C
Shipment<\/strong>: Dry Ice
Cat. No<\/strong>.: LG9751
For Research Use Only.
Not for use in diagnostic procedures.<\/p>\n<\/div>\n\n\n\n

\n
\"
HCD Extraction Kit\n<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n

\u062d\u0630\u0641 \u0646\u0627\u062e\u0627\u0644\u0635 \u0647\u0627\u06cc \u0646\u0627\u0634\u06cc \u0627\u0632 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0645\u06cc\u0632\u0628\u0627\u0646 \u06a9\u0647 \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u0646\u062f\u0647\n\u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0628\u06cc\u0648\u062a\u06a9\u0646\u0648\u0644\u0648\u0698\u06cc \u0647\u0633\u062a\u0646\u062f\u060c \u06cc\u06a9 \u0645\u0631\u062d\u0644\u0647 \u0636\u0631\u0648\u0631\u06cc \u062f\u0631 \u062a\u0648\u0644\u06cc\u062f \u062f\u0627\u0631\u0648\u0647\u0627 \u0645\u06cc \u0628\u0627\u0634\u062f. \u06cc\u06a9\u06cc \u0627\u0632\n\u0646\u0627\u062e\u0627\u0644\u0635\u06cc \u0647\u0627\u06cc\u06cc \u06a9\u0647 \u0628\u0627\u06cc\u062f \u062f\u0631 \u0645\u0631\u062d\u0644\u0647 \u062a\u062e\u0644\u06cc\u0635 \u062f\u0627\u0631\u0648 \u067e\u0627\u06a9\u0633\u0627\u0632\u06cc \u0634\u0648\u062f\u060c DNA \u0628\u0627\u0642\u06cc \u0645\u0627\u0646\u062f\u0647 \u0627\u0632 \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646\n\u0627\u0633\u062a. \u0645\u0637\u0627\u0628\u0642 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0647\u0627\u06cc \u062a\u0648\u0644\u06cc\u062f \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062f\u0631 \u06a9\u0634\u062a \u0633\u0644\u0648\u0644\u060c \u0645\u0642\u062f\u0627\u0631 DNA \u0645\u06cc\u0632\u0628\u0627\u0646 \u062f\u0631 \u0645\u062d\u0635\u0648\u0644 \u0646\u0647\u0627\u06cc\u06cc \u0628\u0627\u06cc\u062f \u062a\u0627 \u062d\u062f\n\u0627\u0645\u06a9\u0627\u0646 \u06a9\u0627\u0647\u0634 \u06cc\u0627\u0641\u062a\u0647 \u0628\u0627\u0634\u062f \u0648 \u0628\u0647 \u0648\u0633\u06cc\u0644\u0647 \u0631\u0648\u0634\u06cc \u0628\u0627 \u062d\u0633\u0627\u0633\u06cc\u062a \u0628\u0627\u0644\u0627 \u0627\u0646\u062f\u0627\u0632\u0647 \u06af\u06cc\u0631\u06cc \u0634\u0648\u062f. \u0627\u0646\u062f\u0627\u0632\u0647\n\u06af\u06cc\u0631\u06cc DNA \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646 \u06cc\u0627\nHost Cell DNA (HCD)\n\u0628\u0647 \u0631\u0648\u0634\u06cc \u0646\u06cc\u0627\u0632 \u062f\u0627\u0631\u062f \u06a9\u0647 \u0628\u0631 \u0627\u0633\u0627\u0633 \u06af\u0627\u06cc\u062f\u0644\u0627\u06cc\u0646 \u0647\u0627\u06cc ICH \u0645\u0639\u062a\u0628\u0631 \u0634\u062f\u0647 \u0628\u0627\u0634\u062f \u062a\u0627 \u0628\u062a\u0648\u0627\u0646\u062f \u0645\u0642\u062f\u0627\u0631\n\u06a9\u0645\u06cc HCD \u0631\u0627 \u062f\u0631 \u062f\u0627\u0631\u0648\n\u062a\u0639\u06cc\u06cc\u0646 \u06a9\u0646\u062f. \u0627\u0645\u0631\u0648\u0632\u0647 \u0631\u0627\u06cc\u062c \u062a\u0631\u06cc\u0646 \u0631\u0648\u0634 \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0648 \u062a\u0639\u06cc\u06cc\u0646 \u06a9\u0645\u06cc DNA \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646\u060c \u062a\u06a9\u0646\u06cc\u06a9 PCR \u06a9\u0645\u06cc \u06cc\u0627 Quantitative PCR (qPCR) \u0645\u06cc \u0628\u0627\u0634\u062f.<\/p>\n\n\n\n

\u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f<\/strong> \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0627\u0648\u0644\u06cc\u0646 \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u0646\u062f\u0647 \u06a9\u06cc\u062a \u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 HCD \u062f\u0631 \u0627\u06cc\u0631\u0627\u0646\u060c \u0627\u0632 \u0633\u06cc\u0633\u062a\u0645 qPCR \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0628\u0627\u0642\u06cc\u0645\u0627\u0646\u062f\u0647 DNA \u0627\u0632 \u0631\u062f\u0647 \u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc \u06a9\u0647 \u0628\u0647 \u0637\u0648\u0631 \u06af\u0633\u062a\u0631\u062f\u0647 \u062f\u0631 \u062a\u0648\u0644\u06cc\u062f \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062f\u0627\u0631\u0648\u06cc\u06cc \u0628\u0647 \u06a9\u0627\u0631 \u0645\u06cc \u0631\u0648\u0646\u062f\u060c \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u06a9\u0646\u062f. \u0628\u0631\u0627\u06cc \u0627\u06cc\u0646 \u0645\u0646\u0638\u0648\u0631 \u06a9\u06cc\u062a \u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 HCD \u0628\u0627\u0642\u06cc\u0645\u0627\u0646\u062f\u0647 \u0627\u0632 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0645\u06cc\u0632\u0628\u0627\u0646\u00a0 \u00a0CHO, BHK, SP2\/0, E.coli<\/em> and Yeast \u062a\u0648\u0633\u0637 \u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f \u062a\u0648\u0644\u06cc\u062f \u0645\u06cc \u0634\u0648\u0646\u062f. \u0627\u06cc\u0646 \u06a9\u06cc\u062a \u0647\u0627 (HCD ContaFind Kits)<\/strong> \u062a\u0648\u0627\u0646\u0627\u06cc\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0633\u0631\u06cc\u0639 \u0648 \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0645\u0642\u062f\u0627\u0631 \u06a9\u0645\u06cc DNA \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646 \u0631\u0627 \u062f\u0631 \u062d\u062f \u067e\u06cc\u06a9\u0648\u06af\u0631\u0645 \u062f\u0627\u0631\u0646\u062f.<\/p>\n\n\n\n

<\/p>\n\n\n\n

The removal of host cell impurities is a critical step in the\nproduction of biopharmaceutical products. One impurity targeted for clearance\nduring the purification process is residual DNA arising from host cells. The\nregulatory guidance for products produced in cell culture specifies that DNA\ncontent in the final product should be as low as possible, as determined by a\nhighly sensitive method. Measurement of Host Cell DNA (HCD) will require an\nassay validated to the ICH Guidelines for quantitative measurement. There are a\nnumber of methods which can be used to measure Host cell DNA. Today the most\ncommon means of detecting and quantitating Host Cell DNA is by Quantitative PCR\n(qPCR) technology.Livogen\nPharmed Co.<\/strong> as the first producer of HCD detection kits in\nIran, applied a qPCR-based system for the detection of residual DNA from CHO,\nBHK, SP2\/0, E.coli<\/em> and Yeast cell lines, widely used cell lines for\nproduction of biopharmaceutical products. The HCD ContaFind Kits<\/strong> enables\nrapid and specific quantification of pictogram levels of Host Cell DNA\n\n\n\n<\/p>\n","protected":false},"excerpt":{"rendered":"

Quantity: 50 reactions Buffer Storage : 2-8 \u00b0C Shipment: Ice gel Lysis Mix Storage: -20 \u00b0C Shipment: Dry Ice Cat. No.: LG9751 For Research Use Only. Not for use in diagnostic procedures. \u062d\u0630\u0641 \u0646\u0627\u062e\u0627\u0644\u0635 \u0647\u0627\u06cc \u0646\u0627\u0634\u06cc \u0627\u0632 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0645\u06cc\u0632\u0628\u0627\u0646 \u06a9\u0647 \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u0646\u062f\u0647 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0628\u06cc\u0648\u062a\u06a9\u0646\u0648\u0644\u0648\u0698\u06cc \u0647\u0633\u062a\u0646\u062f\u060c \u06cc\u06a9 \u0645\u0631\u062d\u0644\u0647 \u0636\u0631\u0648\u0631\u06cc \u062f\u0631 \u062a\u0648\u0644\u06cc\u062f \u062f\u0627\u0631\u0648\u0647\u0627 \u0645\u06cc \u0628\u0627\u0634\u062f. \u06cc\u06a9\u06cc […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[],"class_list":["post-823","post","type-post","status-publish","format-standard","hentry","category-1"],"_links":{"self":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/posts\/823","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/comments?post=823"}],"version-history":[{"count":0,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/posts\/823\/revisions"}],"wp:attachment":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/media?parent=823"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/categories?post=823"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/tags?post=823"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}