{"id":821,"date":"2019-12-03T08:03:10","date_gmt":"2019-12-03T09:03:10","guid":{"rendered":"http:\/\/livogen.co\/?p=821"},"modified":"2020-02-23T05:26:30","modified_gmt":"2020-02-23T06:26:30","slug":"hcd-e-coli-contafind-kit","status":"publish","type":"post","link":"https:\/\/livogen.co\/fa\/2019\/12\/03\/hcd-e-coli-contafind-kit\/","title":{"rendered":"HCD (E.coli) ContaFind Kit (Probe) DNA Detection Kit"},"content":{"rendered":"\n<div class=\"wp-block-columns has-2-columns is-layout-flex wp-container-core-columns-is-layout-7387b849 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"wp-block-paragraph\" dir=\"ltr\"><strong>Quantity<\/strong>: 50 reactions <br><strong>Store<\/strong>: -20 \u00b0C <br><strong>Cat<\/strong>. No.: LG9402 <br><strong>Shipment<\/strong>: Dry ice For Research Use Only. <br>Not for use in diagnostic procedures.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image\"><img fetchpriority=\"high\" decoding=\"async\" width=\"764\" height=\"540\" src=\"http:\/\/livogen.co\/wp-content\/uploads\/2019\/12\/Picture1.png\" alt=\"HCD (E.coli) ContaFind Kit (Probe) DNA Detection Kit\" class=\"wp-image-822\" srcset=\"https:\/\/livogen.co\/wp-content\/uploads\/2019\/12\/Picture1.png 764w, https:\/\/livogen.co\/wp-content\/uploads\/2019\/12\/Picture1-600x424.png 600w, https:\/\/livogen.co\/wp-content\/uploads\/2019\/12\/Picture1-300x212.png 300w, https:\/\/livogen.co\/wp-content\/uploads\/2019\/12\/Picture1-260x185.png 260w, https:\/\/livogen.co\/wp-content\/uploads\/2019\/12\/Picture1-705x498.png 705w\" sizes=\"(max-width: 764px) 100vw, 764px\" \/><figcaption>HCD (E.coli) ContaFind Kit (Probe)\nDNA Detection Kit\n<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p class=\"wp-block-paragraph\">\u062d\u0630\u0641 \u0646\u0627\u062e\u0627\u0644\u0635 \u0647\u0627\u06cc \u0646\u0627\u0634\u06cc \u0627\u0632 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0645\u06cc\u0632\u0628\u0627\u0646 \u06a9\u0647 \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u0646\u062f\u0647\n\u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0628\u06cc\u0648\u062a\u06a9\u0646\u0648\u0644\u0648\u0698\u06cc \u0647\u0633\u062a\u0646\u062f\u060c \u06cc\u06a9 \u0645\u0631\u062d\u0644\u0647 \u0636\u0631\u0648\u0631\u06cc \u062f\u0631 \u062a\u0648\u0644\u06cc\u062f \u062f\u0627\u0631\u0648\u0647\u0627 \u0645\u06cc \u0628\u0627\u0634\u062f. \u06cc\u06a9\u06cc \u0627\u0632\n\u0646\u0627\u062e\u0627\u0644\u0635\u06cc \u0647\u0627\u06cc\u06cc \u06a9\u0647 \u0628\u0627\u06cc\u062f \u062f\u0631 \u0645\u0631\u062d\u0644\u0647 \u062a\u062e\u0644\u06cc\u0635 \u062f\u0627\u0631\u0648 \u067e\u0627\u06a9\u0633\u0627\u0632\u06cc \u0634\u0648\u062f\u060c DNA \u0628\u0627\u0642\u06cc \u0645\u0627\u0646\u062f\u0647 \u0627\u0632 \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646\n\u0627\u0633\u062a. \u0645\u0637\u0627\u0628\u0642 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0647\u0627\u06cc \u062a\u0648\u0644\u06cc\u062f \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062f\u0631 \u06a9\u0634\u062a \u0633\u0644\u0648\u0644\u060c \u0645\u0642\u062f\u0627\u0631 DNA \u0645\u06cc\u0632\u0628\u0627\u0646 \u062f\u0631 \u0645\u062d\u0635\u0648\u0644 \u0646\u0647\u0627\u06cc\u06cc \u0628\u0627\u06cc\u062f \u062a\u0627 \u062d\u062f\n\u0627\u0645\u06a9\u0627\u0646 \u06a9\u0627\u0647\u0634 \u06cc\u0627\u0641\u062a\u0647 \u0628\u0627\u0634\u062f \u0648 \u0628\u0647 \u0648\u0633\u06cc\u0644\u0647 \u0631\u0648\u0634\u06cc \u0628\u0627 \u062d\u0633\u0627\u0633\u06cc\u062a \u0628\u0627\u0644\u0627 \u0627\u0646\u062f\u0627\u0632\u0647 \u06af\u06cc\u0631\u06cc \u0634\u0648\u062f. \u0627\u0646\u062f\u0627\u0632\u0647\n\u06af\u06cc\u0631\u06cc DNA \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646 \u06cc\u0627\nHost Cell DNA (HCD)\n\u0628\u0647 \u0631\u0648\u0634\u06cc \u0646\u06cc\u0627\u0632 \u062f\u0627\u0631\u062f \u06a9\u0647 \u0628\u0631 \u0627\u0633\u0627\u0633 \u06af\u0627\u06cc\u062f\u0644\u0627\u06cc\u0646 \u0647\u0627\u06cc ICH \u0645\u0639\u062a\u0628\u0631 \u0634\u062f\u0647 \u0628\u0627\u0634\u062f \u062a\u0627 \u0628\u062a\u0648\u0627\u0646\u062f \u0645\u0642\u062f\u0627\u0631\n\u06a9\u0645\u06cc HCD \u0631\u0627 \u062f\u0631 \u062f\u0627\u0631\u0648\n\u062a\u0639\u06cc\u06cc\u0646 \u06a9\u0646\u062f. \u0627\u0645\u0631\u0648\u0632\u0647 \u0631\u0627\u06cc\u062c \u062a\u0631\u06cc\u0646 \u0631\u0648\u0634 \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0648 \u062a\u0639\u06cc\u06cc\u0646 \u06a9\u0645\u06cc DNA \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646\u060c \u062a\u06a9\u0646\u06cc\u06a9 PCR \u06a9\u0645\u06cc \u06cc\u0627 Quantitative PCR (qPCR) \u0645\u06cc \u0628\u0627\u0634\u062f.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>\u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f<\/strong> \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0627\u0648\u0644\u06cc\u0646 \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u0646\u062f\u0647 \u06a9\u06cc\u062a \u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 HCD \u062f\u0631 \u0627\u06cc\u0631\u0627\u0646\u060c \u0627\u0632 \u0633\u06cc\u0633\u062a\u0645 qPCR \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0628\u0627\u0642\u06cc\u0645\u0627\u0646\u062f\u0647 DNA \u0627\u0632 \u0631\u062f\u0647 \u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc \u06a9\u0647 \u0628\u0647 \u0637\u0648\u0631 \u06af\u0633\u062a\u0631\u062f\u0647 \u062f\u0631 \u062a\u0648\u0644\u06cc\u062f \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062f\u0627\u0631\u0648\u06cc\u06cc \u0628\u0647 \u06a9\u0627\u0631 \u0645\u06cc \u0631\u0648\u0646\u062f\u060c \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u06a9\u0646\u062f. \u0628\u0631\u0627\u06cc \u0627\u06cc\u0646 \u0645\u0646\u0638\u0648\u0631 \u06a9\u06cc\u062a \u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 HCD \u0628\u0627\u0642\u06cc\u0645\u0627\u0646\u062f\u0647 \u0627\u0632 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0645\u06cc\u0632\u0628\u0627\u0646&nbsp; &nbsp;CHO, BHK, SP2\/0, <em>E.coli<\/em> and Yeast \u062a\u0648\u0633\u0637 \u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f \u062a\u0648\u0644\u06cc\u062f \u0645\u06cc \u0634\u0648\u0646\u062f. \u0627\u06cc\u0646 \u06a9\u06cc\u062a \u0647\u0627 <strong>(HCD ContaFind Kits)<\/strong> \u062a\u0648\u0627\u0646\u0627\u06cc\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0633\u0631\u06cc\u0639 \u0648 \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0645\u0642\u062f\u0627\u0631 \u06a9\u0645\u06cc DNA \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646 \u0631\u0627 \u062f\u0631 \u062d\u062f \u067e\u06cc\u06a9\u0648\u06af\u0631\u0645 \u062f\u0627\u0631\u0646\u062f.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\" dir=\"ltr\">The removal of host cell impurities is a critical step in the\nproduction of biopharmaceutical products. One impurity targeted for clearance\nduring the purification process is residual DNA arising from host cells. The\nregulatory guidance for products produced in cell culture specifies that DNA\ncontent in the final product should be as low as possible, as determined by a\nhighly sensitive method. Measurement of Host Cell DNA (HCD) will require an\nassay validated to the ICH Guidelines for quantitative measurement. There are a\nnumber of methods which can be used to measure Host cell DNA. Today the most\ncommon means of detecting and quantitating Host Cell DNA is by Quantitative PCR\n(qPCR) technology.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\" dir=\"ltr\"><strong>Livogen Pharmed Co.<\/strong> as the first producer of HCD detection kits in Iran, applied a qPCR-based system for the detection of residual DNA from CHO, BHK, SP2\/0, <em>E.coli<\/em> and Yeast cell lines, widely used cell lines for production of biopharmaceutical products. The <strong>HCD ContaFind Kits<\/strong> enables rapid and specific quantification of picogram levels of Host Cell DNA.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Quantity: 50 reactions Store: -20 \u00b0C Cat. No.: LG9402 Shipment: Dry ice For Research Use Only. Not for use in diagnostic procedures. \u062d\u0630\u0641 \u0646\u0627\u062e\u0627\u0644\u0635 \u0647\u0627\u06cc \u0646\u0627\u0634\u06cc \u0627\u0632 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0645\u06cc\u0632\u0628\u0627\u0646 \u06a9\u0647 \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u0646\u062f\u0647 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0628\u06cc\u0648\u062a\u06a9\u0646\u0648\u0644\u0648\u0698\u06cc \u0647\u0633\u062a\u0646\u062f\u060c \u06cc\u06a9 \u0645\u0631\u062d\u0644\u0647 \u0636\u0631\u0648\u0631\u06cc \u062f\u0631 \u062a\u0648\u0644\u06cc\u062f \u062f\u0627\u0631\u0648\u0647\u0627 \u0645\u06cc \u0628\u0627\u0634\u062f. \u06cc\u06a9\u06cc \u0627\u0632 \u0646\u0627\u062e\u0627\u0644\u0635\u06cc \u0647\u0627\u06cc\u06cc \u06a9\u0647 \u0628\u0627\u06cc\u062f \u062f\u0631 \u0645\u0631\u062d\u0644\u0647 \u062a\u062e\u0644\u06cc\u0635 \u062f\u0627\u0631\u0648 \u067e\u0627\u06a9\u0633\u0627\u0632\u06cc [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[57,31],"tags":[],"class_list":["post-821","post","type-post","status-publish","format-standard","hentry","category-----dna---","category-31"],"_links":{"self":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/posts\/821","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/comments?post=821"}],"version-history":[{"count":0,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/posts\/821\/revisions"}],"wp:attachment":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/media?parent=821"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/categories?post=821"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/tags?post=821"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}