{"id":285,"date":"2019-10-06T11:20:08","date_gmt":"2019-10-06T11:20:08","guid":{"rendered":"http:\/\/livogen.co\/?p=285"},"modified":"2020-04-07T05:37:16","modified_gmt":"2020-04-07T05:37:16","slug":"hcd-sp2-0-kit","status":"publish","type":"post","link":"https:\/\/livogen.co\/fa\/2019\/10\/06\/hcd-sp2-0-kit\/","title":{"rendered":"HCD SP2\/0 kit"},"content":{"rendered":"\n

Cat. No.<\/strong>: LG9841 – HCD SP2\/0 kit<\/strong><\/p>\n\n\n\n

\u06a9\u06cc\u062a \u06a9\u0645\u06cc\u062a \u0633\u0646\u062c\u06cc DNA  \u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0628\u0633\u06cc\u0627\u0631 \u062f\u0642\u06cc\u0642 SP2\/0 DNA \u062f\u0631 \u0646\u0638\u0631 \u06af\u0631\u0641\u062a\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a \u0648  \u0627\u06cc\u0646 \u0627\u062c\u0627\u0632\u0647 \u0631\u0627 \u0645\u06cc \u062f\u0647\u062f \u062a\u0627 \u0628\u0627 \u062d\u062c\u0645 \u0627\u0646\u062f\u06a9 \u0646\u0645\u0648\u0646\u0647 \u0646\u062a\u0627\u06cc\u062c \u062f\u0642\u06cc\u0642 \u0628\u062f\u0633\u062a \u0622\u06cc\u062f. \u0645\u062d\u062f\u0648\u062f\u0647 \u062e\u0637\u06cc \u06af\u0633\u062a\u0631\u062f\u0647 \u0641\u0646\u200c\u0622\u0648\u0631\u06cc qPCR  \u0627\u06cc\u0646 \u0627\u062c\u0627\u0632\u0647 \u0631\u0627 \u0645\u06cc\u062f\u0647\u062f \u06a9\u0647 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc\u06cc \u0628\u0627 \u0633\u0637\u0648\u062d \u0645\u062e\u062a\u0644\u0641SP2 \/ 0 DNA  \u060c \u0645\u0627\u0646\u0646\u062f \u0646\u0645\u0648\u0646\u0647 \u0647\u0627 \u062f\u0631 \u062d\u0627\u0644 \u0641\u0631\u0627\u06cc\u0646\u062f \u0628\u0627 \u0645\u0642\u0627\u062f\u06cc\u0631 \u0628\u0627\u0644\u0627\u06cc\u06cc \u0627\u0632 DNA  \u062a\u0627  \u0627\u0633\u062a\u0648\u06a9\u200c\u0647\u0627\u06cc \u062f\u0627\u0631\u0648\u06cc\u06cc  \u0628\u0627 \u0645\u0642\u0627\u062f\u06cc\u0631 \u0628\u0633\u06cc\u0627\u0631 \u06a9\u0645 DNA \u0645\u0648\u0631\u062f \u0622\u0632\u0645\u0648\u0646 \u0642\u0631\u0627\u0631 \u06af\u06cc\u0631\u0646\u062f.<\/p>\n\n\n\n

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Quantity<\/strong>: 50 reactions
Store<\/strong>: -20 \u00b0C
Cat. No.<\/strong>: LG9841
Shipment<\/strong>: Dry ice
For Research Use Only. Not for use in diagnostic procedures.<\/p>\n<\/div>\n\n\n\n

\n
\"\u06a9\u06cc\u062a
\u06a9\u06cc\u062a \u062a\u0634\u062e\u06cc\u0635 DNA \u0645\u06cc\u0632\u0628\u0627\u0646 | HCD (Sp20) ContaFind Kit DNA Detection Kit<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n
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<\/p>\n<\/div>\n\n\n\n

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Sensitivity <\/strong><\/p>\n<\/div>\n<\/div>\n\n\n\n

In order to determine the sensitivity of each Real-time PCR reaction, a dilution series must be prepared from 10 ng\/\u03bcl standard mix down to 0.01 pg\/\u03bcl and be used as Real-time PCR template. The LOD was 1 fg DNA per each Real-time PCR reaction (0.6 pg\/ml) and The LOQ was 3 fg DNA per each Real-time PCR reaction (1.76 pg\/ml). <\/p>\n\n\n\n

<\/p>\n\n\n\n

\u062d\u0630\u0641 \u0646\u0627\u062e\u0627\u0644\u0635 \u0647\u0627\u06cc \u0646\u0627\u0634\u06cc \u0627\u0632 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0645\u06cc\u0632\u0628\u0627\u0646 \u06a9\u0647 \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u0646\u062f\u0647\n\u0645\u062d\u0635\u0648\u0644\u0627\u062a \u0628\u06cc\u0648\u062a\u06a9\u0646\u0648\u0644\u0648\u0698\u06cc \u0647\u0633\u062a\u0646\u062f\u060c \u06cc\u06a9 \u0645\u0631\u062d\u0644\u0647 \u0636\u0631\u0648\u0631\u06cc \u062f\u0631 \u062a\u0648\u0644\u06cc\u062f \u062f\u0627\u0631\u0648\u0647\u0627 \u0645\u06cc \u0628\u0627\u0634\u062f. \u06cc\u06a9\u06cc \u0627\u0632\n\u0646\u0627\u062e\u0627\u0644\u0635\u06cc \u0647\u0627\u06cc\u06cc \u06a9\u0647 \u0628\u0627\u06cc\u062f \u062f\u0631 \u0645\u0631\u062d\u0644\u0647 \u062a\u062e\u0644\u06cc\u0635 \u062f\u0627\u0631\u0648 \u067e\u0627\u06a9\u0633\u0627\u0632\u06cc \u0634\u0648\u062f\u060c DNA \u0628\u0627\u0642\u06cc \u0645\u0627\u0646\u062f\u0647 \u0627\u0632 \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646\n\u0627\u0633\u062a. \u0645\u0637\u0627\u0628\u0642 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0647\u0627\u06cc \u062a\u0648\u0644\u06cc\u062f \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062f\u0631 \u06a9\u0634\u062a \u0633\u0644\u0648\u0644\u060c \u0645\u0642\u062f\u0627\u0631 DNA \u0645\u06cc\u0632\u0628\u0627\u0646 \u062f\u0631 \u0645\u062d\u0635\u0648\u0644 \u0646\u0647\u0627\u06cc\u06cc \u0628\u0627\u06cc\u062f \u062a\u0627 \u062d\u062f\n\u0627\u0645\u06a9\u0627\u0646 \u06a9\u0627\u0647\u0634 \u06cc\u0627\u0641\u062a\u0647 \u0628\u0627\u0634\u062f \u0648 \u0628\u0647 \u0648\u0633\u06cc\u0644\u0647 \u0631\u0648\u0634\u06cc \u0628\u0627 \u062d\u0633\u0627\u0633\u06cc\u062a \u0628\u0627\u0644\u0627 \u0627\u0646\u062f\u0627\u0632\u0647 \u06af\u06cc\u0631\u06cc \u0634\u0648\u062f. \u0627\u0646\u062f\u0627\u0632\u0647\n\u06af\u06cc\u0631\u06cc DNA \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646 \u06cc\u0627\nHost Cell DNA (HCD)\n\u0628\u0647 \u0631\u0648\u0634\u06cc \u0646\u06cc\u0627\u0632 \u062f\u0627\u0631\u062f \u06a9\u0647 \u0628\u0631 \u0627\u0633\u0627\u0633 \u06af\u0627\u06cc\u062f\u0644\u0627\u06cc\u0646 \u0647\u0627\u06cc ICH \u0645\u0639\u062a\u0628\u0631 \u0634\u062f\u0647 \u0628\u0627\u0634\u062f \u062a\u0627 \u0628\u062a\u0648\u0627\u0646\u062f \u0645\u0642\u062f\u0627\u0631\n\u06a9\u0645\u06cc HCD \u0631\u0627 \u062f\u0631 \u062f\u0627\u0631\u0648\n\u062a\u0639\u06cc\u06cc\u0646 \u06a9\u0646\u062f. \u0627\u0645\u0631\u0648\u0632\u0647 \u0631\u0627\u06cc\u062c \u062a\u0631\u06cc\u0646 \u0631\u0648\u0634 \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0648 \u062a\u0639\u06cc\u06cc\u0646 \u06a9\u0645\u06cc DNA \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646\u060c \u062a\u06a9\u0646\u06cc\u06a9 PCR \u06a9\u0645\u06cc \u06cc\u0627 Quantitative PCR (qPCR) \u0645\u06cc \u0628\u0627\u0634\u062f.<\/p>\n\n\n\n

\u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f<\/strong> \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0627\u0648\u0644\u06cc\u0646 \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u0646\u062f\u0647 \u06a9\u06cc\u062a \u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 HCD \u062f\u0631 \u0627\u06cc\u0631\u0627\u0646\u060c \u0627\u0632 \u0633\u06cc\u0633\u062a\u0645 qPCR \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0628\u0627\u0642\u06cc\u0645\u0627\u0646\u062f\u0647 DNA \u0627\u0632 \u0631\u062f\u0647 \u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc \u06a9\u0647 \u0628\u0647 \u0637\u0648\u0631 \u06af\u0633\u062a\u0631\u062f\u0647 \u062f\u0631 \u062a\u0648\u0644\u06cc\u062f \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062f\u0627\u0631\u0648\u06cc\u06cc \u0628\u0647 \u06a9\u0627\u0631 \u0645\u06cc \u0631\u0648\u0646\u062f\u060c \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc \u06a9\u0646\u062f. \u0628\u0631\u0627\u06cc \u0627\u06cc\u0646 \u0645\u0646\u0638\u0648\u0631 \u06a9\u06cc\u062a \u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 HCD \u0628\u0627\u0642\u06cc\u0645\u0627\u0646\u062f\u0647 \u0627\u0632 \u0633\u0644\u0648\u0644 \u0647\u0627\u06cc \u0645\u06cc\u0632\u0628\u0627\u0646   CHO, BHK, SP2\/0, E.coli<\/em> and Yeast \u062a\u0648\u0633\u0637 \u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f \u062a\u0648\u0644\u06cc\u062f \u0645\u06cc \u0634\u0648\u0646\u062f. \u0627\u06cc\u0646 \u06a9\u06cc\u062a \u0647\u0627 (HCD ContaFind Kits)<\/strong> \u062a\u0648\u0627\u0646\u0627\u06cc\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0633\u0631\u06cc\u0639 \u0648 \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0645\u0642\u062f\u0627\u0631 \u06a9\u0645\u06cc DNA \u0633\u0644\u0648\u0644 \u0645\u06cc\u0632\u0628\u0627\u0646 \u0631\u0627 \u062f\u0631 \u062d\u062f \u067e\u06cc\u06a9\u0648\u06af\u0631\u0645 \u062f\u0627\u0631\u0646\u062f.<\/p>\n\n\n\n

The removal of host cell impurities is a critical step in the\nproduction of biopharmaceutical products. One impurity targeted for clearance\nduring the purification process is residual DNA arising from host cells. The\nregulatory guidance for products produced in cell culture specifies that DNA\ncontent in the final product should be as low as possible, as determined by a\nhighly sensitive method. Measurement of Host Cell DNA (HCD) will require an\nassay validated to the ICH Guidelines for quantitative measurement. There are a\nnumber of methods which can be used to measure Host cell DNA. Today the most\ncommon means of detecting and quantitating Host Cell DNA is by Quantitative PCR\n(qPCR) technology.<\/p>\n\n\n\n

Livogen Pharmed Co.<\/strong> as the first\nproducer of HCD detection kits in Iran, applied a qPCR-based system for the\ndetection of residual DNA from CHO, BHK, SP2\/0, E.coli<\/em> and Yeast cell\nlines, widely used cell lines for production of biopharmaceutical products. The\nHCD ContaFind Kits<\/strong> enables rapid and specific quantification of\npictogram levels of Host Cell DNA.<\/p>\n","protected":false},"excerpt":{"rendered":"

Cat. No.: LG9841 – HCD SP2\/0 kit \u06a9\u06cc\u062a \u06a9\u0645\u06cc\u062a \u0633\u0646\u062c\u06cc DNA  \u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0628\u0633\u06cc\u0627\u0631 \u062f\u0642\u06cc\u0642 SP2\/0 DNA \u062f\u0631 \u0646\u0638\u0631 \u06af\u0631\u0641\u062a\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a \u0648  \u0627\u06cc\u0646 \u0627\u062c\u0627\u0632\u0647 \u0631\u0627 \u0645\u06cc \u062f\u0647\u062f \u062a\u0627 \u0628\u0627 \u062d\u062c\u0645 \u0627\u0646\u062f\u06a9 \u0646\u0645\u0648\u0646\u0647 \u0646\u062a\u0627\u06cc\u062c \u062f\u0642\u06cc\u0642 \u0628\u062f\u0633\u062a \u0622\u06cc\u062f. \u0645\u062d\u062f\u0648\u062f\u0647 \u062e\u0637\u06cc \u06af\u0633\u062a\u0631\u062f\u0647 \u0641\u0646\u200c\u0622\u0648\u0631\u06cc qPCR  \u0627\u06cc\u0646 \u0627\u062c\u0627\u0632\u0647 \u0631\u0627 \u0645\u06cc\u062f\u0647\u062f \u06a9\u0647 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc\u06cc \u0628\u0627 \u0633\u0637\u0648\u062d \u0645\u062e\u062a\u0644\u0641SP2 \/ […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[57,31],"tags":[],"class_list":["post-285","post","type-post","status-publish","format-standard","hentry","category-----dna---","category-31"],"_links":{"self":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/posts\/285","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/comments?post=285"}],"version-history":[{"count":0,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/posts\/285\/revisions"}],"wp:attachment":[{"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/media?parent=285"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/categories?post=285"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/livogen.co\/fa\/wp-json\/wp\/v2\/tags?post=285"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}