{"id":9409,"date":"2023-10-25T06:03:14","date_gmt":"2023-10-25T06:03:14","guid":{"rendered":"https:\/\/livogen.co\/?page_id=9409"},"modified":"2023-10-25T07:46:26","modified_gmt":"2023-10-25T07:46:26","slug":"%d9%85%d8%b4%d8%ae%d8%b5%d9%87-%db%8c%d8%a7%d8%a8%db%8c-%d8%a8%d8%a7%d9%86%da%a9-%d9%87%d8%a7%db%8c-%d8%b3%d9%84%d9%88%d9%84%db%8c-%d9%88-%d9%88%db%8c%d8%b1%d9%88%d8%b3%db%8c","status":"publish","type":"page","link":"https:\/\/livogen.co\/fa\/%d9%85%d8%b4%d8%ae%d8%b5%d9%87-%db%8c%d8%a7%d8%a8%db%8c-%d8%a8%d8%a7%d9%86%da%a9-%d9%87%d8%a7%db%8c-%d8%b3%d9%84%d9%88%d9%84%db%8c-%d9%88-%d9%88%db%8c%d8%b1%d9%88%d8%b3%db%8c\/","title":{"rendered":"\u0645\u0634\u062e\u0635\u0647 \u06cc\u0627\u0628\u06cc \u0628\u0627\u0646\u06a9 \u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc \u0648 \u0648\u06cc\u0631\u0648\u0633\u06cc"},"content":{"rendered":"\t\t
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\u0645\u0634\u062e\u0635\u0647 \u06cc\u0627\u0628\u06cc \u0628\u0627\u0646\u06a9 \u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc \u0648 \u0648\u06cc\u0631\u0648\u0633\u06cc<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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<\/a><\/p>

<\/a>Mammalian, Microbial and insect cell bank<\/p>

\u0622\u0632\u0645\u0648\u0646\u200c\u0647\u0627\u06cc \u0645\u0634\u062e\u0635\u0647 \u06cc\u0627\u0628\u06cc \u0628\u0627\u0646\u06a9\u00ad\u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc \u0628\u0647 \u062f\u0644\u06cc\u0644\u00a0 \u062a\u0627\u062b\u06cc\u0631 \u0645\u0633\u062a\u0642\u06cc\u0645 \u0646\u062a\u0627\u06cc\u062c \u0622\u0646\u0647\u0627 \u062f\u0631 \u0633\u0631\u0646\u0648\u0634\u062a \u062f\u0627\u0631\u0648\u06cc \u062a\u0648\u0644\u06cc\u062f\u06cc\u060c \u0628\u0627\u06cc\u062f \u0645\u0637\u0627\u0628\u0642 \u0628\u0627 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644\u200c \u0647\u0627\u06cc \u0633\u0627\u0632\u0645\u0627\u0646\u200c\u0647\u0627\u06cc \u0646\u0638\u0627\u0631\u062a\u06cc \u0628\u06cc\u0646 \u0627\u0644\u0645\u0644\u0644\u06cc (ICH \u060c FDA \u0648 WHO) \u0648 \u062f\u0631 \u0633\u0637\u062d \u0628\u0647\u06cc\u0646\u0647\u00ad\u0627\u06cc \u0627\u0632 \u0646\u0638\u0631 \u06a9\u06cc\u0641\u06cc\u062a \u062a\u062c\u0647\u06cc\u0632\u0627\u062a\u060c \u0645\u0648\u0627\u062f \u0648 \u0631\u0648\u0634 \u0648 \u0622\u0646\u0627\u0644\u06cc\u0632 \u0627\u0646\u062c\u0627\u0645 \u0634\u0648\u0646\u062f. \u0627\u06cc\u0646 \u0622\u0632\u0645\u0648\u0646\u200c\u0647\u0627 \u0628\u0647 \u0637\u0648\u0631 \u0645\u0639\u0645\u0648\u0644 \u0628\u0631\u0627\u06cc \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc\u060c (Master Cell Bank (MCB\u060c Working Cell Bank (WCB) \u0648 (EoPCB) End of Production Cell Bank \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc\u200c\u06af\u06cc\u0631\u062f. \u062f\u0631 \u062d\u0627\u0644 \u062d\u0627\u0636\u0631\u060c \u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f \u062e\u062f\u0645\u0627\u062a \u0645\u0634\u062e\u0635\u0647 \u06cc\u0627\u0628\u06cc \u0631\u0627 \u0628\u0631\u0627\u06cc \u0628\u0627\u0646\u06a9\u200c\u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc \u062c\u0627\u0646\u0648\u0631\u06cc (CHO, HEK\u060cVero \u060cBHK \u0648 SP2\/0)\u060c \u0628\u0627\u0646\u06a9\u200c\u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc \u062d\u0634\u0631\u0627\u062a (High Five \u0648 SF9)\u060c \u0628\u0627\u0646\u06a9\u200c\u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc \u0628\u0627\u06a9\u062a\u0631\u06cc\u0627\u06cc\u06cc ( Escherichia coli ) \u0648 \u0628\u0627\u0646\u06a9\u200c\u0647\u0627\u06cc \u0633\u0644\u0648\u0644\u06cc \u0645\u062e\u0645\u0631 (Pichia Pastoris) \u0631\u0627\u0647 \u0627\u0646\u062f\u0627\u0632\u06cc \u0646\u0645\u0648\u062f\u0647 \u0648 \u062f\u0631 \u062d\u0627\u0644 \u062a\u0648\u0633\u0639\u0647 \u0627\u06cc\u0646 \u062e\u062f\u0645\u0627\u062a \u0645\u06cc\u200c\u0628\u0627\u0634\u062f. \u0627\u06cc\u0646 \u062e\u062f\u0645\u0627\u062a \u0647\u0645\u0631\u0627\u0647 \u0628\u0627 \u06af\u0632\u0627\u0631\u0634 \u06a9\u0627\u0645\u0644 \u0648 \u06af\u0648\u0627\u0647\u06cc \u0622\u0646\u0627\u0644\u06cc\u0632 \u0645\u0639\u062a\u0628\u0631 \u0627\u0631\u0627\u0626\u0647 \u0645\u06cc\u200c\u0634\u0648\u0646\u062f.<\/p>

<\/a>Viral Cell Bank Characterization<\/p>

\u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f \u062e\u062f\u0645\u0627\u062a \u0645\u0634\u062e\u0635\u0647\u00ad\u06cc\u0627\u0628\u06cc \u0628\u0627\u0646\u06a9\u200c\u0647\u0627\u06cc \u0648\u06cc\u0631\u0648\u0633\u06cc \u0631\u0627 \u0628\u0647 \u062f\u0644\u06cc\u0644 \u0627\u0647\u0645\u06cc\u062a \u0622\u0646\u0647\u0627 \u062f\u0631 \u0645\u0647\u0646\u062f\u0633\u06cc \u0698\u0646\u062a\u06cc\u06a9 \u0648 \u06a9\u0627\u0631\u0628\u0631\u062f \u0634\u0627\u0646 \u062f\u0631 \u0627\u0646\u062a\u0642\u0627\u0644 \u0698\u0646 \u0628\u0627 \u0627\u0647\u062f\u0627\u0641 \u0633\u0644\u0648\u0644 \u062f\u0631\u0645\u0627\u0646\u06cc \u0648 \u0698\u0646 \u062f\u0631\u0645\u0627\u0646\u06cc\u060c \u0628\u0631\u0627\u0633\u0627\u0633 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644\u200c\u0647\u0627\u06cc \u0628\u06cc\u0646 \u0627\u0644\u0645\u0644\u0644\u06cc \u0627\u0646\u062c\u0627\u0645 \u062f\u0627\u062f\u0647 \u0648 \u06af\u0648\u0627\u0647\u06cc \u0622\u0646\u0627\u0644\u06cc\u0632 \u0645\u0639\u062a\u0628\u0631 \u0627\u0631\u0627\u0626\u0647 \u0645\u06cc\u200c\u0646\u0645\u0627\u06cc\u062f. \u0628\u0627\u0646\u06a9\u00ad\u0647\u0627\u06cc \u0648\u06cc\u0631\u0648\u0633\u06cc \u0634\u0627\u0645\u0644 Master Virus Bank(MVB) \u060c (Working Virus Bank(WVB \u0648 End-of-Production Virus Bank (EoPVB) \u0645\u06cc \u0628\u0627\u0634\u0646\u062f.<\/p>

\u0639\u0644\u0627\u0648\u0647 \u0628\u0631 \u062e\u062f\u0645\u0627\u062a \u0641\u0648\u0642\u060c \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f \u0642\u0627\u062f\u0631 \u0627\u0633\u062a \u062a\u0627 \u0645\u06cc\u0632\u0627\u0646 \u0628\u0642\u0627\u06cc\u0627\u06cc \u0698\u0646\u0648\u0645 \u0645\u06cc\u0632\u0628\u0627\u0646 \u0647\u0627\u06cc \u0630\u06a9\u0631 \u0634\u062f\u0647 (HCD) \u0648 \u0647\u0645\u0686\u0646\u06cc\u0646 \u0645\u06cc\u0632\u0628\u0627\u0646 \u06a9\u0644\u0633\u062a\u0631\u06cc\u062f\u06cc\u0648\u0645\u00a0 \u0628\u0648\u062a\u0648\u0644\u06cc\u0648\u0645 \u0631\u0627 \u0628\u0647 \u0634\u06a9\u0644 \u062e\u062f\u0645\u0627\u062a \u0628\u0647 \u0645\u0634\u062a\u0631\u06cc\u0627\u0646 \u0627\u0631\u0627\u0626\u0647 \u062f\u0647\u062f.<\/p>

\u062e\u062f\u0645\u0627\u062a \u0645\u0634\u062e\u0635\u0647\u00ad\u06cc\u0627\u0628\u06cc \u0634\u0627\u0645\u0644 \u0622\u0632\u0645\u0648\u0646\u00ad\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0647\u0648\u06cc\u062a \u0698\u0646\u062a\u06cc\u06a9\u06cc(Identity)\u060c \u062a\u0634\u062e\u06cc\u0635 \u0622\u0644\u0648\u062f\u06af\u06cc (Purity)\u060c\u062a\u0634\u062e\u06cc\u0635 \u067e\u0627\u06cc\u062f\u0627\u0631\u06cc \u0698\u0646\u062a\u06cc\u06a9\u06cc (Stability) \u0648 \u062a\u0634\u062e\u06cc\u0635 \u0622\u0644\u0648\u062f\u06af\u06cc\u00ad\u0647\u0627\u06cc \u0648\u06cc\u0631\u0648\u0633\u06cc(Viral Safety) \u0645\u06cc\u00ad\u0628\u0627\u0634\u062f.<\/p>

Mammalian, Insects and Viral Cell Bank Characterization<\/p>

Identity<\/p><\/td>

Mammalian and Insect Cell Bank<\/p><\/td><\/tr>

DNA Barcoding Analysis<\/p><\/td>

DNA Fingerprinting<\/p><\/td>

Species-Specific PCR\/Real-Time PCR<\/p><\/td><\/tr><\/tbody><\/table>

\u00a0<\/p>

Identity<\/p><\/td>

Microbial Cell Bank<\/p><\/td><\/tr>

Determination of Cellular Morphology by the Gram Staining<\/p><\/td>

Determination of Viability by the CFU<\/p><\/td>

16s rRNA<\/p><\/td><\/tr><\/tbody><\/table>

\u00a0<\/p>

Identity<\/p><\/td>

Virus Bank<\/p><\/td><\/tr>

Virus Titration by Real-Time PCR<\/p><\/td>

Virus Identity by Species-Specific PCR\/Real-Time PCR<\/p><\/td><\/tr><\/tbody><\/table>

\u00a0<\/p>

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Mammalian, Insects and Virus Cell Bank Characterization<\/p>

\u00a0<\/p>

Purity<\/p><\/td>

Mammalian and Insect Cell Bank<\/p><\/td><\/tr>

Sterility) Bacteria and Fungi Contamination Detection)<\/p><\/td>

Mycoplasma Contamination Detection (Real-time PCR, PCR)<\/p><\/td>

Mycoplasma Contamination Detection (Culture method and Indicator cells)<\/p><\/td><\/tr>

Spiroplasma Contamination Detection (real-time PCR)*<\/p><\/td>

Mycobacterium Contamination by PCR<\/p><\/td>

Mycobacterium Contamination Detection by Culture<\/p><\/td><\/tr><\/tbody><\/table>

*\u0627\u06cc\u0646 \u062e\u062f\u0645\u062a \u0641\u0642\u0637 \u0628\u0631\u0627\u06cc \u0628\u0627\u0646\u06a9 \u0633\u0644\u0648\u0644\u06cc \u062d\u0634\u0631\u0627\u062a \u062a\u0648\u0635\u06cc\u0647 \u0645\u06cc\u00ad\u06af\u0631\u062f\u062f.<\/p>

\u00a0<\/p>

Purity<\/p><\/td>

Microbial Cell Bank<\/p><\/td><\/tr>

Detection of Lytic and Lysogenic Bacteriophage by Plaque Assay<\/p><\/td><\/tr><\/tbody><\/table>

\u00a0<\/p>

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Mammalian, Insects, Microbial and Virus Cell Bank Characterization<\/p>

Stability<\/p><\/td>

Genetic Stability Assessment<\/p><\/td>

\u00a0<\/p><\/td><\/tr>

Transgene Insertion Copy number by qPCR<\/p><\/td>

DNA\/RNA Sequencing Analysis<\/p><\/td>

Restriction Map Analysis<\/p><\/td>

\u00a0<\/p><\/td><\/tr>

DNA\/RNA Stability<\/p><\/td>

Retention of Selectable Marker<\/p><\/td>

Karyotyping<\/p><\/td>

\u00a0<\/p><\/td><\/tr><\/tbody><\/table>

Mammalian, Insects and Virus Cell Bank Characterization<\/p>

\u00a0<\/p>

Viral Safety<\/p><\/td>

Retrovirus Detection<\/p><\/td><\/tr>

Transmission Electron Microscopy (TEM)<\/p><\/td>

Infectivity<\/p><\/td>

Reverse Transcriptase Assay using PERT Assay<\/p><\/td><\/tr>

Reverse Transcriptase Assay using ELISA<\/p><\/td><\/tr>

Testing the Presence of Adventitious Viruses by In vivo Assay<\/p><\/td><\/tr>

Adult mice<\/p><\/td>

Suckling mice<\/p><\/td>

Embryonated eggs<\/p><\/td><\/tr>

Testing the Presence of Adventitious Viruses by In vitro Assay<\/p><\/td><\/tr>

28-day In vitro Assay for Presence of Viral Contamination using 3 cell lines or more<\/p><\/td>

14-day In vitro Assay for Presence of Viral Contamination using 3 cell lines or more<\/p>

\u00a0<\/p><\/td><\/tr>

Antibody Production Test<\/p><\/td><\/tr>

Mouse Antibody Production Test (MAP)<\/p><\/td>

Hamster Antibody Production Test (HAP)<\/p><\/td>

Rat Antibody Production Test (RAP)<\/p><\/td><\/tr>

Detection of Virus Contamination by q-PCR<\/p><\/td><\/tr>

Arbovirus<\/p><\/td>

Noda Virus*<\/p><\/td>

Bovine Viruses<\/p><\/td>

Porcine Viruses<\/p><\/td>

MMV Virus<\/p><\/td>

Human Viruses<\/p><\/td><\/tr>

\u00a0<\/td>\u00a0<\/td>\u00a0<\/td>\u00a0<\/td>\u00a0<\/td>\u00a0<\/td>\u00a0<\/td>\u00a0<\/td>\u00a0<\/td><\/tr><\/tbody><\/table>

<\/a>*\u0627\u06cc\u0646 \u062e\u062f\u0645\u062a \u0641\u0642\u0637 \u0628\u0631\u0627\u06cc \u0628\u0627\u0646\u06a9 \u0633\u0644\u0648\u0644\u06cc \u062d\u0634\u0631\u0627\u062a \u062a\u0648\u0635\u06cc\u0647 \u0645\u06cc\u00ad\u06af\u0631\u062f\u062f.<\/p>

<\/a>\u0645\u0634\u062e\u0635\u0647 \u00ad\u06cc\u0627\u0628\u06cc \u0633\u0644\u0648\u0644\u00ad\u0647\u0627\u06cc \u0628\u0646\u06cc\u0627\u062f\u06cc<\/h1>

Stem cells characterization<\/p>

\u0627\u0645\u0631\u0648\u0632\u0647 \u0633\u0644\u0648\u0644\u200c\u0647\u0627\u06cc \u0628\u0646\u06cc\u0627\u062f\u06cc \u0627\u0632 \u062c\u0627\u06cc\u06af\u0627\u0647 \u0648\u06cc\u0698\u0647\u00ad\u0627\u06cc \u062f\u0631 \u062a\u062d\u0642\u06cc\u0642\u0627\u062a \u0632\u06cc\u0633\u062a \u0641\u0646\u0627\u0648\u0631\u06cc \u067e\u0632\u0634\u06a9\u06cc \u0648 \u0645\u0647\u0646\u062f\u0633\u06cc \u0628\u0627\u0641\u062a \u0628\u0631\u062e\u0648\u0631\u062f\u0627\u0631 \u0647\u0633\u062a\u0646\u062f. \u0628\u0627 \u062a\u0648\u062c\u0647 \u0628\u0647 \u06a9\u0627\u0631\u0628\u0631\u062f \u0631\u0648\u0632 \u0627\u0641\u0632\u0648\u0646 \u0622\u0646\u0647\u0627 \u062f\u0631 \u062f\u0631\u0645\u0627\u0646 \u0628\u06cc\u0645\u0627\u0631\u06cc\u200c\u0647\u0627\u06cc \u0645\u062e\u062a\u0644\u0641 \u0648 \u0644\u0632\u0648\u0645 \u0645\u0634\u062e\u0635\u0647\u00ad\u06cc\u0627\u0628\u06cc \u0622\u0646\u0647\u0627 \u0628\u0631\u0627\u0633\u0627\u0633 \u0631\u0648\u0634\u200c\u0647\u0627\u06cc \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f\u060c \u0634\u0631\u06a9\u062a \u0644\u06cc\u0648\u0698\u0646 \u0641\u0627\u0631\u0645\u062f \u062e\u062f\u0645\u0627\u062a \u0645\u0634\u062e\u0635\u0647\u00ad\u06cc\u0627\u0628\u06cc \u0633\u0644\u0648\u0644\u200c\u0647\u0627\u06cc \u0628\u0646\u06cc\u0627\u062f\u06cc \u0631\u0627 \u0645\u0637\u0627\u0628\u0642 \u0628\u0627 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644\u200c\u0647\u0627\u06cc \u0633\u0627\u0632\u0645\u0627\u0646\u200c\u0647\u0627\u06cc \u0646\u0638\u0627\u0631\u062a\u06cc \u0628\u06cc\u0646 \u0627\u0644\u0645\u0644\u0644\u06cc \u0648 \u0647\u0645\u0631\u0627\u0647 \u0628\u0627 \u06af\u0632\u0627\u0631\u0634 \u06a9\u0627\u0645\u0644 \u0648 \u06af\u0648\u0627\u0647\u06cc \u0622\u0646\u0627\u0644\u06cc\u0632 \u0645\u0639\u062a\u0628\u0631 \u0627\u0631\u0627\u0626\u0647 \u0645\u06cc\u200c\u0646\u0645\u0627\u06cc\u062f.<\/p>

\u00a0<\/p>

Functional Characterization of Stem Cells<\/p><\/td><\/tr>

Differentiation Potential (Osteogenesis, Chondrogenesis and Adipogenesis assay)<\/p><\/td>

Surface Markers Evaluation<\/p><\/td><\/tr>

Cell Proliferation Assay<\/p><\/td>

Purity (content of non-MSC populations\/nonviable cells)<\/p><\/td><\/tr><\/tbody><\/table>

\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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